本团队运用生态学前沿技术（如高通量16S/18s扩增子测序、宏基因组测序、宏转录组测序及数据分析）进行深入的微生物响应环境变化和污染物胁迫的分子机制研究，为环境治理与生态安全提供科学支撑。研究方向聚焦在以下三个方面：1）光合自养微生物的光合作用抗逆机制；2）环境微生物互作调控机制；3）环境微生物定向进化。在相关领域，团队成员先后承担或参与多项国家、省自然科学基金项目，发表高水平SCI论文28篇，获得国家或国际发明专利5项。
二、团队成员
徐魁，博士，副教授，硕士研究生，研究领域为环境微生物互作调控机制。曾获留学基金委项目资助到加拿大魁北克大学攻读博士学位。博士毕业后先后在加拿大和国内重点实验室工作，运用生态学前沿技术（如高通量16S/18S扩增子测序、宏基因组测序、宏转录组测序及数据分析）进行深入的微生物响应环境变化和污染物胁迫的分子机制研究。主要研究领域为：原绿球藻的光合抗逆机制及其与共存异养微生物的互作调控机制。在相关领域，先后承担两项省自然科学基金面上项目。在Environmental Pollution, Aquatic Toxicology, Journal of Hazardous Materials等学术期刊上发表SCI论文26篇。
陈春，博士，讲师，硕士生导师，研究领域为环境微生物定向进化。
三、研究内容
团队重点研究集中在以下几个方面：
（1）光合自养微生物的光合作用抗逆机制
原绿球藻是地球上丰度最高的光合自养微生物，其通过光合作用释放的氧气占大气氧气含量的10%，对维持大气氧气和二氧化碳含量稳定起重要作用，生态意义重大。原绿球藻根据其在水体中的垂直分布可分为高光型原绿球藻和低光型原绿球藻。低光型原绿球藻不能在高光环境下生长的机制一直不清楚，是光合抗逆研究领域的热点科学问题。本团队通过研究原绿球藻光合关键蛋白含量、基因表达水平和PSII活性变化等，发现低光型原绿球藻对高光敏感是因为其清除PsbA蛋白的速率低导致的，揭示了原绿球藻抗逆新机制。
（2）环境微生物互作调控机制
水域生态系统中，真核微生物和细菌存在广泛相互作用，但这种相互作用机制如何维持生态系统平衡仍不清楚。本团队通过高通量测序技术分析水域生态系统中微生物间相互作用，发现真核微生物与细菌间相互作用普遍且重要，同时发掘了相互作用体系中关键物种。这些研究结果对理解水体微生物互作提供了关键基础信息，并对监测或调节水体生态系统提供潜在微生物种类。
（3）环境微生物定向进化
微生物代谢产物海藻糖具有生命之糖美誉，拥有高度安全性和有良好稳定性，对生物体或生物大分子具有优良的非特异性保护作用，被广泛应用于医药、食品等领域。本团队通过基因数据库挖掘、微生物定向进化等技术手段，将制备海藻糖的酶热稳定性以及海藻糖转化率大幅度提高，为工业大规模生产制备海藻糖提供技术基础和理论依据。
四、承担科研项目
（1）湖北省自然科学基金面上项目：高光型原绿球藻光系统II捕光色素抗氧化胁迫保护机制，2023-2025
（2）广东省自然科学基金面上项目：纳米颗粒物对蓝细菌光合机理的影响机制，2018-2023
（3）湖北师范大学高层次人才引进科研启动经费：典型新型污染物对蓝细菌光合作用复合影响机制研究，2021-2024
（4）湖北师范大学高层次人才引进科研启动经费：麦芽寡糖基海藻糖水解酶热稳定性改造研究，2021-2024
（5）食用野生植物保育与利用湖北省重点实验室开放课题基金：典型食用野生植物与其附生异养微生物互作的分子机制研究，2023-2025
五、学术论文
[1] Daxi Wang, Xiang Gao, Xiaojiao Wang, Xiaolong Yuan, Xinhong Guo, Yong Zhang, Kui Xu, Zhengke Li. 2024. Diverse thermal responses of the growth, photosynthesis, lipid and fatty acids in the terrestrial oil-producing microalga vischeria sp. Wl1. Journal of applied Phycology 36: 29-39.
[2] Jiacong Shen, Xiafei Zheng, Minhai Liu, Kui Xu, Lin He, Zhihua Lin. 2024. Targeted cultivation of diatoms in mariculture wastewater by nutrient regulation and UV-C irradiance. Frontiers in Microbiology 15: 1371855.
[3] Yong Zhang, Yong Zhang, Shuai Ma, Hanbing Chen, Jiabing Li, Zhengke Li, Kui Xu, Ruiping Huang, Hong Zhang, Yonghe Han, Jun Sun. 2023. Reallocation of elemental content and macromolecules in the coccolithophore Emiliania huxleyi to acclimate to climate change. Biogeosciences 20: 1299-1312.
[4] Xiafei Zheng, Kui Xu, Jonathan Naoum, Yingli Lian, Bo Wu, Zhili He, Qingyun Yan. 2023. Deciphering microeukaryotic-bacterial co-occurrence networks in coastal aquaculture ponds. Maine Life Science and Technology 5: 44-55.
[5] Xueqing Yang, Xiaoli Yu, Qiang He, Ting Deng, Xiaotong Guan, Yingli Lian, Kui Xu, Longfei Shu, Cheng Wang, Qingyun Yan, Yuchun Yang, Bo Wu, Zhili He. Niche differentitation among comammox (Nitrospira inopinata) and other metabolically distinct nitrifiers. Frontiers in Microbiology 13: 956860.
[6] Hu Jihong, Chen G, Xu Kui, Wang Jun. 2022. Cadmium in cereal crops: uptake and transport mechanisms and minimizing strategies. Journal of Agricultural and Food Chemistry 70: 5961-5974.
[7] Kui Xu, Zhou Li, Philippe Juneau, Fanshu Xiao, Yingli Lian, Wei Zhang, Longfei Shu, Haibo Jiang, Keke Zhang, Cheng Wang, Shanquan, Wang, Qingyun Yan, Zhili He. 2021. Toxic and protective mechanisms of cyanobacterium Synechocystis sp. in response to titanium dioxide nanoparticles. Environmental Pollution. Doi: 10.1016/j.envpol.2021.116508.
[8] Huang Yu, Xiafei Zheng, Wanlin Weng, Xizhe Yan, Pubo Chen, Xingyu Liu, Tao Peng, Qiuping Zhong, Kui Xu, Cheng Wang, Longfei Shu, Tony Yang, Fanshu Xiao, Zhili He, Qingyun Yan. 2021. Synergistic effects of antimony and arsenic contaminations on bacterial, archaeal and fungal communities in the rhizosphere of Miscanthus sinensis: insights for nitrification and carbon mineralization. Journal of Hazardous Materials. DOI: 10.1016/j.jhazmat.2021.125094.
[9] Libin Zhao*, Kui Xu*#, Philippe Juneau, Peihuan Huang, Yingli Lian, Xiafei Zheng, Qiuping Zhong, Wei Zhang, Fanshu Xiao, Bo Wu, Qingyun Yan, Zhili He#. 2021. Light modulates the effect of antibiotic norfloxacin on photosynthetic processes of Microcystis aeruginosa. Aquatic Toxicology. Doi: 10.1016/j.aquatox.2021.105826.
[10] Emei Xie, Kui Xu, Zhengke Li, Wei Li, Xiangqi Yi, Hongzhou Li, Yonghe Han, Hong Zhang, Yong Zhang. 2021. Disentangling the effects of ocean carbonation and acidification on elemental contents and macromolecules of the Coccolithophore Emiliania huxleyi. Frontiers in Microbiology.
[11] Pubo Chen, Jie Huang, Liuyu Rao, Wengen Zhu, Yuhe Yu, Fanshu Xiao, Xiaojuan Chen, Huang Yu, Yongjie Wu,Kui Xu, Xiafei Zheng, Ruiwen Hu, Zhili He, Qingyun Yan. 2021. Resistance and resilience of fish gut microbiota to silver nanoparticles. mSystems.
[12] 陈春;宿玲恰;夏伟;吴敬;定向进化提高来源于Arthrobacter ramosus的MTHase的热稳定性,生物技术通报 , 2021, 37(3): 84-91
[13] Xiaoli Yu, Xueqin Yang, Yongjie Wu, Yisheng Peng, Tony Yang, Fanshu Xiao, Qiuping Zhong, Kui Xu, Longfei Shu, Qiang He, Yun Tian, Qingyun Yan, Cheng Wang, Bo Wu, Zhili He. 2020. Sonneratia apetala introduction alters methane cycling microbial communities and increase methane emissions in mangrove ecosystems. Soil Biology and Biochemistry 144: 107775.
[14] Xingyu Liu, Chao Yang, Xiaoli Yu, Huang Yu, Wei Zhuang, Hang Gu, Kui Xu, Xiafei Zheng, Cheng Wang, Fanshu Xiao, Bo Wu, Zhili He, Qingyun Yan. 2020. Revealing structure and assembly for rizophyte-endophyte diazotrophic community in mangrove ecosystem after introduced Sonneratia apetala and Laguncularia racemose. Science of the Total Environment 721: 137807.
[15] Yongjie Wu, Fanshu Xiao, Cheng Wang, Longfei Shu, Xiafei Zheng, Kui Xu, Xiaoli Yu, Keke Zhang, Hongtian Luo, Yufeng Yang, Zhili He, Qingyun Yan. 2020. The beta-diversity of Siganus fuscescens-associated Microbial communities from different habitats increases with body weight. Frontiers in Microbiology. Doi: 10.3389/fmicb.2020.01562.
[16] Huang Yu, Xizhe Yan, Xiafei Zheng, Kui Xu, Qiuping Zhong, Tong Yang, Feifei Liu, Cheng Wang, Longfei Shu, Zhili He, Fanshu Xiao, Qingyun Yan. 2020. Differential distribution of and similar biochemical responses to different species of arsenic and antimony in Vetiveria zizanioides. Environmental Geochemistry and Health. Doi:10.1007/s10653-020-00658-4.
[17] Zhengke Li, Guozheng Dai, Yong Zhang, Kui Xu, Laura Bretherton, Zoe V. Finkel, Andrew J. Irwin, Philippe Juneau, Baosheng Qiu. 2020. Photosynthetic adaptation to light availability shapes the ecological success of bloom-forming cyanobacterium Pseudanabaena to iron limitation. Journal of Phycology. Doi/10.1111/jpy.13040.
[18] 赵立斌，徐魁#，连英丽，颜庆云，贺志理#。2020。原绿球藻对环境胁迫的生理和分子响应机制。微生物学报。Doi: 10.13343/j.cnki.wsxb.20200413.
[19] Zhou Li*, Philippe Juneau, Yingli Lian, Wei Zhang, Shanquan Wang, Cheng Wang, Longfei Shu, Qingyun Yan, Zhili He#,Kui Xu*#. 2020.Effects of titanium dioxide nanoparticles on photosynthetic and antioxidative processes of Scenedesmus obliquus. Plants.
[20] Chen Chun; Lingqia Su; Lian Wu; Jiahai Zhou; Jing Wu; Enhanced the catalytic efficiency and thermostability of maltooligosyltrehalose synthase from Arthrobacter ramosus by directed evolution, Biochemical Engineering Journal, 2020, 162: 107724-107724
[21] Kui Xu*, Francis Racine*, Zhili He, Philippe Juneau. 2019. Impacts of hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor (mesotrione) on photosynthetic processes in Chlamydomonas reinhardtii. Environmental Pollution. 244: 295-303.
[22] Chun Chen; Lingqia Su; Fei Xu; Yongmei Xia; Jing Wu; Improved thermostability of maltooligosyltrehalose synthase from Arthrobacter ramosus by directed evolution and site-directed mutagenesis. Journal of Agricultural and Food Chemistry, 2019, 67(19): 5595
[23] Kui Xu, Johann Lavaud, Rupert Perkins, Emily Austen, Marlene Bonnanfant, Douglas A. Campbell. 2018. Phytoplankton σPSII and excitation dissipation; Implications for estimates of primary productivity. Frontiers in Marine Science. Doi: 10.3389/fmars.2018.00281
[24] Erin Bonisteel, Brooke Turner, Cole Murphy, Jenna-Rose Melanson, Nicole Duff, Brian Beardsall, Kui Xu, Douglas Campbell, Amanda Cockshutt. 2018. Strain specific differences in rates of Photosynthesis II repair in picocyanobacteria correlate to differences in FtsH protein levels and isoform expression patterns. Plos One. Doi/10.1371/journal.pone.0209115.
[25] Cole D. Murphy, Guangyan Ni, Gang Li, Audrey Barnett, Kui Xu, Jessica Grant-Burt, Justin D. Liefer, David J. Suggett, Douglas A. Campbell. 2017. Quantitating active Photosystem II reaction center content from fluorescence induction transients. Limnology and Oceanography: Methods. 15: 54-69.
[26] Kui Xu, Zhengke Li, Shuwen Liu and Baosheng Qiu. 2017. Effects of iron deficiency on the growth and photosynthesis of three bloom-forming cyanobacterial species isolated from lake Taihu. Phycological Research. 65: 151-159.
[27] Kui Xu, Jessica Grant-Burt, Natalie Donaher, Douglas A. Campbell. 2017. Connectivity among Photosystem II centers in Phytoplankters: Patterns and Responses. BBA-Bioenergetics. 1858: 459-474.
[28] Kui Xu, Philippe Juneau. 2016. Different physiological and photosynthetic responses of three cyanobacterial strains to light and zinc. Aquatic Toxicology 170: 251-258.
[29] Kui Xu, Zhengke Li, Baosheng Qiu and Philippe Juneau. 2013. Different responses to high light stress of toxic and non-toxic Microcystis aeruginosa acclimated under two light intensities and zinc concentrations. Toxicological and Environmental Chemistry 95: 1145-1156.
[30] Kui Xu, Haibo Jiang, Philippe Juneau and Baosheng Qiu. 2012. Comparative studies on the photosynthetic responses of three freshwater phytoplankton to temperature and light regimes. Journal of Applied Phycology. 24: 1113-1122.
六、发明专利
（1）吴敬; 宿玲恰; 陈春; Maltooligosyl Trehalose Synthase Mutant with Improved Thermal Stability, 美国, US10865405B2
（2）吴敬; 宿玲恰; 陈春;一种海藻糖转化率提高的麦芽寡糖基海藻糖合成酶突变体及其应用,中国, CN202110573135.7
（3）吴敬; 宿玲恰; 陈春;一种热稳定性提高的麦芽寡糖基海藻糖合成酶突变体, 中国, CN201810568782.7
（4）吴敬; 宿玲恰; 陈春; 一种热稳定性和海藻糖产量提高的MTSase突变体, 中国, CN201810568791.6
（5）吴敬; 宿玲恰; 陈春;一种热稳定性提高的MTSase突变体及其应用, 中国, CN201910318019.3
